LOCUS       EF1a-RLuc-T2A-RF        6771 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
            This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     LTR             6537..6771
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    5858..6454
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             5450..5848
                     /label=Bsd
     RBS             4842..5426
                     /label=IRES
     CDS             4079..4132
                     /label=T2A
     CDS             4133..4837
                     /label=RFP
     CDS             3134..4063
                     /label=Renilla\Luciferase\(RLuc)
BASE COUNT     1627 a      1723 c      1914 g      1507 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccgggt 
     3121 tggatccgcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac 
     3181 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta 
     3241 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc 
     3301 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc 
     3361 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga 
     3421 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt 
     3481 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa 
     3541 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg 
     3601 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct 
     3661 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc 
     3721 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac 
     3781 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat 
     3841 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga 
     3901 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac 
     3961 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg 
     4021 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gaggctagcg gcgatatcga 
     4081 gggcagaggc agcctgctga catgcggaga tgtggaagag aaccccgggc ccatggtgtc 
     4141 caagggcgag gaagtgatca aagagttcat gcggttcaaa gtgcggatgg aaggcagcat 
     4201 gaacggccac gagttcgaga tcgagggcga gggcgaaggc agaccttacg agggaaccca 
     4261 gaccgccaag ctgaaagtga ccaagggcgg acccctgccc ttcgcctggg atattctgag 
     4321 cccccagttt atgtacggca gcaaagccta cgtgaagcac cccgccgaca tccccgacta 
     4381 caagaagctg agcttccccg agggcttcaa gtgggagaga gtgatgaact tcgaggacgg 
     4441 cggcctcgtg accgtgaccc aggatagctc tctgcaggac ggcaccctga tctacaaagt 
     4501 gaagatgcgg ggcaccaact tcccccccga tggccccgtg atgcagaaaa agaccatggg 
     4561 ctgggaggcc agcaccgaga gactgtaccc tagagatggc gtgctgaagg gcgagatcca 
     4621 ccaggccctg aagctgaagg atggcggcca ctacctggtg gagttcaaga ccatctacat 
     4681 ggccaagaaa cccgtgcagc tgcccggcta ctactacgtg gacaccaagc tggacatcac 
     4741 cagccacaac gaggactaca ccatcgtgga acagtacgag cggagcgagg gccggcacca 
     4801 cctgtttctg tatggcatgg acgagctgta caagtgaact agcccctctc cctccccccc 
     4861 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 
     4921 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 
     4981 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 
     5041 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 
     5101 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 
     5161 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 
     5221 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 
     5281 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt 
     5341 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg 
     5401 aaaaacacga tgataatatg gccacactag agatccaccg gtcgccacca tggccaagcc 
     5461 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc 
     5521 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac 
     5581 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac 
     5641 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag 
     5701 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat 
     5761 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct 
     5821 gccctctggt tatgtgtggg agggctaagc ggccgcgtcg acaatcaacc tctggattac 
     5881 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 
     5941 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 
     6001 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 
     6061 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 
     6121 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 
     6181 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     6241 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 
     6301 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 
     6361 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 
     6421 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac 
     6481 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     6541 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 
     6601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     6661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     6721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc a 
//
